Methodology

A number of protocols are available for potential clients. Please contact Lyda Mimi Brown, Technical Director of the facility at lbrown@bsd.uchicago.edu for protocols.

Read about The Microwestern Array approach from Nature Methods.

For further reference, the Jones Laboratory has provided a series of short videos documenting the MWA procedure step-by-step from gel fabrication to blot analysis.

Pouring the MWA gel, part I

 

Pouring the MWA gel, part II

 

Storing the MWA gels

 

Preparing MWA samples and gels

 

Arraying procedure, part I

 

Arraying procedure, part II

 

MWA electrophoresis step

 

MWA Transfer Step

 

Adding primary antibody to MWA Blot, part I

 

Adding primary antibody to MWA Blot, part II

 

Adding secondary antibody to MWA Blot, part I

 

Adding secondary antibody to MWA Blot, part II

 

Washing secondary antibody from MWA blots

 

Scanning MWA blots

 

MWA blot analysis

Contact Information

Please direct questions and comments concerning the MWAC to Lyda Mimi Brown, Research Technologist of the facility at lbrown@bsd.uchicago.edu

Microwestern Array Core Facility (MWAC)
The University of Chicago
Knapp Center for Biomedical Discovery
Room 10126
900 E. 57th St. Chicago, IL 60637

Phone: 773.702.8651
Fax: 773.834.2877