Matt Milton

Alumni, Research Technician,
- Kevin White Laboratory

Contact Information

Institute for Genomics and Systems Biology
The University of Chicago
KCBD Room 10240D
900 E. 57th St.
Chicago, IL, 60637

Phone: (773) 834-0074
Email: .(JavaScript must be enabled to view this email address)

Bio

My primary responsibility is to generate K562 cells stably expressing bacterial artificial chromosomes (BACs) for the ENCyclopedia of DNA Elements (ENCODE) project. Each BAC is engineered to tag a single transcription factor with GFP. After the BAC has been transfected, the expression of the fusion protein is validated via western blot and immunofluorescence staining.  Upon passing both validations, the transcription factors are fixed to DNA for chromatin immunoprecipitation followed by sequencing (ChIP-seq).  Identifying and cataloging the transcription factor binding sites for the known 1800 human transcription factors using ChIP-seq will provide a detailed repository to enable future experiments.


Research Papers