- Director, Advanced Imaging Core (AIC)
Knapp Center for Biomedical Discovery
900 E. 57th Street
Chicago, IL 60637
Phone: (773) 834-0074
Dr. Dmitri Novikov is the Technical Director of the IGSB’s advances imaging core. He is responsible for the Digital Scanned Laser Light Sheet Fluorescence Microscope (DSLM), acquired from the European Molecular Biology Laboratory. This microscope takes advantage of a new optical arrangement using one objective for specimen illumination generating a thin optical sheet, and another objective for collecting the image data at various angles. This way, phototoxicity is reduced a hundred folds and more, and high resolution, fast live imaging of Drosophila embryonic development and other life processes becomes an exciting possibility
Dr. Dmitri Novikov studying chromatin structure and function using polytene chromosomes of the fruit fly Drosophila melanogaster. The exceptional organization of the polytene chromosomes has made them a unique model system for visualization of genes. Indeed, only in these naturally “magnified” chromosomes can we distinguish sequences as short as several kilobase pairs as well as relate them to specific band and interband structures and study their transcriptional dynamics. A major barrier to full exploitation of polytene chromosome cytology has been the difficulty in producing good chromosome preparations. Dr. Novikov is using his new method applying high pressure and other modifications to overcome this barrier. Light microscopy images of such slides compared to electron microscopy images for the first time show similar or superior detail.