Systems analysis of EGF receptor signaling dynamics with microwestern arrays
Nature Methods
Systems analysis of EGF receptor signaling dynamics with microwestern arrays
Mark F Ciaccio, Joel P Wagner, Chih-Pin Chuu, Douglas A Lauffenburger & Richard B Jones
Published online: 24 January 2010 | doi:10.1038/nmeth.1418
Microwestern arrays combine the advantages of scalability of reverse phase protein arrays and the information content of western blotting for analyzing protein abundance and modification state with high sensitivity and throughput. The method is demonstrated for analyzing phosphorylation state changes in the EGF receptor signaling network using Bayesian network modeling.
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