Deciphering the Contribution of Cis-regulatory Variants to Gene Expression Patterns


Joshua Rest, University of Chicago


February 20, 2007, 12:30 pm – 2:30 pm


Bldg. 221, Conference Room A216, Argonne National Laboratory


Gene expression is controlled by transcription factors that bind to a variable family of motifs in a gene’s promoter; however the role of variability within motif families has not been systematically studied. Deciphering the function of these variable positions is important for understanding the complex cis-regulatory code that underlies the physiology, ecology and evolution of gene expression. Using a computational approach, I show that functional variants of these motifs among genes within the yeast genome are associated with condition-specific differential expression. The regulatory consequences of these variants are often conserved across different yeast species, and their evolution is associated with change in gene expression. The power of binding site variants to predict gene expression is tested by experimentally switching between binding site variants through site-directed mutagenesis in yeast.

Latest News

Start-up founded by IGSB faculty wins $250,000 Polsky Center award

BiomeSense, a startup developing biosensors that can detect particular kinds of bacteria in patients’ feces that could help improve the efficacy of clinical trials, won the University of Chicago’s Innovation Fund finals and an investment of up to $250,000 from the college.

Massive data analysis shows what drives the spread of flu in the US

Models built with data from health claims, weather, geography and Twitter predict how the flu spreads from the south and southeastern coast

Subscribe to RSS Feed